Journal of Endocrinology and Metabolism, ISSN 1923-2861 print, 1923-287X online, Open Access
Article copyright, the authors; Journal compilation copyright, J Endocrinol Metab and Elmer Press Inc
Journal website http://www.jofem.org

Original Article

Volume 5, Number 1-2, April 2015, pages 172-178


Evaluation of Bone Tissue After Cafeteria-Diet-Induced Obesity and Periodontitis in Rats

Figures

Figure 1.
Figure 1. Representative photomicrography of an animal from control group. D: dentin; P: pulp; EG: gingival epithelium; CO: crest bone; LP: periodontal ligament; C (hematoxylin and eosin, × 40).
Figure 2.
Figure 2. Representative photomicrography of an animal from the group with induced periodontal disease and gingival epithelium exocytosis (*) (hematoxylin and eosin, × 40).
Figure 3.
Figure 3. Representative photomicrography of an animal from the group with induced periodontal disease. D: dentin; P: pulp; CB: bacterial colonies. Exposure of the cervical third of the root (hematoxylin and eosin, × 40).
Figure 4.
Figure 4. Representative photomicrography of an animal from group obese. D: dentin; P: pulp; CB: bacterial colonies (hematoxylin and eosin, × 40).
Figure 5.
Figure 5. Representative photomicrography of bone resorption area in an animal from group obese. Howship’s lacunae occupied by an osteoclast in the bone crest region (hematoxylin and eosin, × 400).
Figure 6.
Figure 6. Representative photomicrography of an animal from the obese group with periodontal disease. D: dentin; EG: gingival epithelium; EJ: junctional epithelium; CB: bacterial colonies (hematoxylin and eosin, × 40).
Figure 7.
Figure 7. Representative photomicrography of an animal from the obese group with periodontal disease. Note that bone crests are tapered (arrow’s head) and adjacent to the buccal root (lower right portion of the image); only the tip of the crest (arrow) is extremely low, the mean/apical level of the root (hematoxylin and eosin, × 40).
Figure 8.
Figure 8. Representative photomicrography of bone neoformation in an animal from the obese group with periodontal disease (hematoxylin and eosin, × 400).

Tables

Table 1. Cafeteria Diet (High-Fat Diet) Composition
 
Energy value (kJ/100 g)Carbohydrates (g/100 g)Proteins (g/100 g)Fat (g/100 g)Sodium (mg/100 g)
Cheetos Snack (Pepsico, Brazil)1,948726.417.2676
Bacon snack/Trofeu (Santa Helena, Brazil)2,200568.8301,040
Cornstarch cookie (Zadimel, Brazil)1,79373810.7300
Chocolate cake (Renata, Brazil)1,79855521.7141.7
Soda/coke (Coca-cola, Brazil)17811005
Soda/Guarana (Antarctica, Brazil)16810005.5
Italian salami (Sadia, Brazil)1,822222381,140
Sausage (Sadia, Brazil)1,5541.416341,342
Bisnaguinha (bread) (Nutrella, Brazil)1,3285411.26.2300
Chocolate Waffer (Bauducco, Brazil)2,17663527113
Bologna (Frimesa, Brazil)845212161,545
Marshmallow (Fini, Brazil)1,423805046

 

Table 2. Body Weight Values, Nasoanal Length and Lee Index
 
GroupsFinal weight (g)Nasoanal length (cm)Lee index (g/cm)
P < 0.01. Different letters indicate that the results are statistically different between groups, in the same parameter of evaluation. The values represent mean ± standard deviation.
CONTROL472 ± 1.0A24.0 ± 1.0A320.7 ± 15.0A
LIG460 ± 2.0A24.0 ± 1.0A316.9 ± 10.0B
OBESE630 ± 5.5B25.5 ± 1.0AB336.0 ± 13.0C
OBESE + LIG618 ± 2.5B26.4 ± 1.0B322.0 ± 11.0D

 

Table 3. Values of the Weights of Retroperitoneal and Periepidimal Fat With Relationship at the Total Weight in Animals
 
GroupsRetroperitoneal fatPeriepidimal fat
Weights (g)Weight percentage (%)Weights (g)Weight percentage (%)
P < 0.01. Different letters indicate that the results are statistically different between groups, in the same parameter of evaluation. The values represent mean ± standard deviation.
CONTROL5.8 ± 1.0A1.2 ± 0.2A7.4 ± 1.4A1.6 ± 0.3A
LIG6.0 ± 1.0A1.3 ± 0.2A7.2 ± 1.0A1.6 ± 0.2A
OBESE30.6 ± 4.0B4.8 ± 0.5B14.3 ± 2.9B2.3 ± 0.4B
OBESE + LIG26.0 ± 4.0B4.2 ± 0.7B14.4 ± 1.8B2.3 ± 0.2B

 

Table 4. Average Counting - Alveolar Bone Osteocytes in Animals
 
GroupsMean
P < 0.01. Different letters indicate that the results are statistically different between groups, in the same parameter of evaluation. The values represent mean ± standard deviation.
CONTROL51.27 ± 1.59A
LIG45.98 ± 1.05B
OBESE48.78 ± 0.89C
OBESE + LIG37.24 ± 0.90D

 

Table 5. Mean Values of the Distance From the Cementoenamel Junction to Alveolar Bone
 
GroupsMean (µm)
P < 0.01. Different letters indicate that the results are statistically different between groups, in the same parameter of evaluation. The values represent mean ± standard deviation.
CONTROL336.85 ± 10.84A
LIG776.19 ± 34.44B
OBESE542.29 ± 12.84C
OBESE + LIG822.12 ± 24.96D