Figures
Figure 1. Time course of the disappearance of paracetamol in rat’s small intestinal perfusate during perfusion of the jejunal loop with isotonic medium containing 500 µM paracetamol. Each value represents the mean of five independent experiments of five rats. No statistical significance was found within the tested parameters. STZ: streptozotocin.
Figure 2. Alteration in the total excreted amount of paracetamol β-D-glucuronide (PG) into the lumen of the small intestine of rat during perfusion of the jejunal loop with isotonic medium containing 500 µM paracetamol. Each value represents the mean of five independent experiments ± standard error. **P < 0.01. STZ: streptozotocin.
Figure 3. Alteration in the total excreted amount of paracetamol sulfate (PS) into the lumen of the small intestine of rat during perfusion of the jejunal loop with isotonic medium containing 500 µM paracetamol. Each value represents the mean of five independent experiments ± standard error. No statistical significance was found within the tested parameters. STZ: streptozotocin.
Figure 4. Alteration in the total excreted amount of paracetamol cysteine (PC) into the lumen of the small intestine of rat during perfusion of the jejunal loop with isotonic medium containing 500 µM paracetamol. Each value represents the mean of five independent experiments ± standard error. **P < 0.01. STZ: streptozotocin.
Figure 5. Alteration in the total excreted amount of paracetamol mercapturate (PM) into the lumen of the small intestine of rat during perfusion of the jejunal loop with isotonic medium containing 500 µM paracetamol. Each value represents the mean of five independent experiments ± standard error. **P < 0.01. STZ: streptozotocin.
Figure 6. HPLC chromatogram of the glutathione (GSH) and oxidized glutathione (GSSG) standards prepared with the blank perfusate. Peak 1 represents the reduced GSH (tR = 2.454 min); peak 2 represents the GSSG (tR = 2.776 min).
Figure 7. Concentration of reduced glutathione (GSH) (the ratio of buffer to the wet tissue was 10:1 (mL/g)) in the small intestine, liver and kidney in control animals. Each value represents the mean of five independent experiments of five rats ± standard error. STZ: streptozotocin.
Figure 8. Concentration of oxidized glutathione (GSSG, the ratio of buffer to the tissue was 10:1 (mL/g)) in the small intestine without and with paracetamol perfusion in case of diabetic rats. Each value represents the mean of five independent experiments ± standard error. **P < 0.01. STZ: streptozotocin.