J Endocrinol Metab

Journal of Endocrinology and Metabolism, ISSN 1923-2861 print, 1923-287X online, Open Access

Article copyright, the authors; Journal compilation copyright, J Endocrinol Metab and Elmer Press Inc

Journal website http://www.jofem.org

Original Article

Volume 7, Number 3, June 2017, pages 77-85

MicroRNAs Responsible for Inflammation in Obesity

**Table 1.** List of GWAS and Meta-Analysis for Initial Data Set
Name	# Individuals	# Male (case/control)	# Female (case/control)	Age min.	Age max.	Analytical method	Related citations
GWAS of adiposity-related heterogeneity in patterns of type II diabetes susceptibility	13,965: initial panel 4,862 (cases: 1,924 and controls: 2,938); second panel 9,103 (cases: 3,757 and controls: 5,346)	Initial panel 2,563 (1,118/1,445); second panel 4,856 (2,137/2,719)	Initial panel 2,299 (806/1,493); second panel 4,247 (1,620/2,627)	39	65	Affymetrix 393,453	Timpson et al [17] Hindorff et al [18]
GWAS of type II diabetes mellitus	6,674 (cases: 1,399 and controls: 5,275)	3,557 (832/2,743)	3,099 (567/2,532)	41	77	Hap300	Steinthorsdottir et al [19] Johnson and O’Donnell [20]
GWAS of waist circumference in individuals of Caucasian descent	70,014: initial panel 31,373; replication panel 38,641	Initial panel 13,993; replication panel 16,769	Initial panel 17,380; replication panel 21,872	45	76	Affymetrix and Illumina up to 512,349	Heard-Costa et al [21] Hindorff et al [18]
GWAS of extreme obesity	3,907 (cases: 775 and controls: 3,132)	1,008 (200/808)	2,899 (575/2,324)	18	75	Illumina 457,251	Cotsapas et al [22] Hindorff et al [18]
GWAS of body mass index	38,759	13,759	25,000	24	81	Affymetrix 490,032	Frayling et al [23] Hindorff et al [18]
GWAS of body mass index in individuals of European descent	16,876	9,121	7,755	31	69	Affymetrix 344,883	Loos et al [24] Hindorff et al [18]
GWAS of body mass index	32,387	16,211	16,176	26	75	Illumina and Affymetrix 2,399,588	Willer et al [25] Hindorff et al [18]
GWAS of body mass index and weight	42,428	17,468	24,960	34	75	Illumina 305,846	Thorleifsson et al [26] Hindorff et al [18]
GWAS of body mass index and waist circumference in the Framingham Heart Study	1,345	619	726	23	41	Affy100,000	Fox et al [27] Johnson and O’Donnell [20] Hindorff et al [18]
GWAS of obesity-related traits	4,298	1,831	2,467	14	102	Affy 10,000 Affy 500,000	Scuteri et al [28] Johnson and O’Donnell [20] Hindorff et al [18]
GWAS of weight and body mass index	4,110	1,777	2,333	30	73	Illumina 318,237	Johansson et al [29] Hindorff et al [18]
GWAS of extreme obesity	26,660: initial panel 5,373 (cases: 2,633 and controls: 2,740); replication panel 21,287	Initial panel 1,465 (673/792); replication panel 11,033	Initial panel 3,908 (1,960/1,948); replication panel 10,254	18	73	Illumina 545,349	Paternoster et al [30] Hindorff et al [18]
GWAS of obesity	2,188: initial panel 1,060 (cases: 520 and controls: 540); replication panel 1,128	Initial panel 40 (32/8); replication panel 45	Initial panel 1,020 (488/532); replication panel 1,083	16	65	Illumina about 550,000	Wang et al [31] Hindorff et al [18]
GWAS of obesity	10,664: initial panel 327 (cases: 164 and controls: 163); replication panel 10,337 (cases: 4,674 and controls: 5,663)	Initial panel 59 (33/26); replication panel 4,126 (1,678/2,448)	Initial panel 268 (131/137); replication panel 6,212 (2,996/3,216)	16	73	Affymetrix 406,177	Jiao et al [32] Hindorff et al [18]
GWAS of body mass index	4,989: initial panel 1,715; replication panel 3,274	Initial panel 714 (354/360); replication panel 1,355	Initial panel 1,001 (545/456); replication panel 1,919	33	72	Affymetrix 746,626	Ng et al [33] Hindorff et al [18]
GWAS of body mass index	249,796	107,795	140,490	14	102	Affymetrix, Illumina and Perlegen about 2.8 million (imputed)	Speliotes et al [34] Hindorff et al [18]
Meta-analysis of extreme obesity	2,258	1,005	1,253	8	32	Affymetrix and Illumina 1,596,878 (imputed)	Scherag et al [35] Hindorff et al [18]
GWAS of adult body mass index in a British population	9,023	4,487	4,536	44	45	Affymetrix GeneChip Mapping 500K Illumina Infinium HumanHap550	Strachan et al [36]
GWAS of obesity	10,391	Randomly selected	Randomly selected	9	80	Illumina 1,283,957 (imputed)	Dorajoo et al [37] Hindorff et al [18]
Meta-analysis of GWAS informative for adult waist circumference and waist-hip ratio	140,644: initial panel 38,580; replication panel 102,064	Randomly selected	Randomly selected	31	73	Affymetrix and Illumina 2,573,738 (imputed)	Lindgren et al [38] Hindorff et al [18]

Table 1. List of GWAS and Meta-Analysis for Initial Data Set

Name

# Individuals

# Male (case/control)

# Female (case/control)

Age min.

Age max.

Analytical method

Related citations

GWAS of adiposity-related heterogeneity in patterns of type II diabetes susceptibility

13,965: initial panel 4,862 (cases: 1,924 and controls: 2,938); second panel 9,103 (cases: 3,757 and controls: 5,346)

Initial panel 2,563 (1,118/1,445); second panel 4,856 (2,137/2,719)

Initial panel 2,299 (806/1,493); second panel 4,247 (1,620/2,627)

Affymetrix 393,453

Timpson et al [17]
Hindorff et al [18]

GWAS of type II diabetes mellitus

6,674 (cases: 1,399 and controls: 5,275)

3,557 (832/2,743)

3,099 (567/2,532)

Hap300

Steinthorsdottir et al [19]
Johnson and O’Donnell [20]

GWAS of waist circumference in individuals of Caucasian descent

70,014: initial panel 31,373; replication panel 38,641

Initial panel 13,993; replication panel 16,769

Initial panel 17,380; replication panel 21,872

Affymetrix and Illumina up to 512,349

Heard-Costa et al [21]
Hindorff et al [18]

GWAS of extreme obesity

3,907 (cases: 775 and controls: 3,132)

1,008 (200/808)

2,899 (575/2,324)

Illumina 457,251

Cotsapas et al [22]
Hindorff et al [18]

GWAS of body mass index

38,759

13,759

25,000

Affymetrix 490,032

Frayling et al [23]
Hindorff et al [18]

GWAS of body mass index in individuals of European descent

16,876

9,121

7,755

Affymetrix 344,883

Loos et al [24]
Hindorff et al [18]

GWAS of body mass index

32,387

16,211

16,176

Illumina and Affymetrix 2,399,588

Willer et al [25]
Hindorff et al [18]

GWAS of body mass index and weight

42,428

17,468

24,960

Illumina 305,846

Thorleifsson et al [26]
Hindorff et al [18]

GWAS of body mass index and waist circumference in the Framingham Heart Study

1,345

619

726

Affy100,000

Fox et al [27]
Johnson and O’Donnell [20]
Hindorff et al [18]

GWAS of obesity-related traits

4,298

1,831

2,467

102

Affy 10,000
Affy 500,000

Scuteri et al [28]
Johnson and O’Donnell [20]
Hindorff et al [18]

GWAS of weight and body mass index

4,110

1,777

2,333

Illumina 318,237

Johansson et al [29]
Hindorff et al [18]

GWAS of extreme obesity

26,660: initial panel 5,373 (cases: 2,633 and controls: 2,740); replication panel 21,287

Initial panel 1,465 (673/792); replication panel 11,033

Initial panel 3,908 (1,960/1,948); replication panel 10,254

Illumina 545,349

Paternoster et al [30]
Hindorff et al [18]

GWAS of obesity

2,188: initial panel 1,060 (cases: 520 and controls: 540); replication panel 1,128

Initial panel 40 (32/8); replication panel 45

Initial panel 1,020 (488/532); replication panel 1,083

Illumina about 550,000

Wang et al [31]
Hindorff et al [18]

GWAS of obesity

10,664: initial panel 327 (cases: 164 and controls: 163); replication panel 10,337 (cases: 4,674 and controls: 5,663)

Initial panel 59 (33/26); replication panel 4,126 (1,678/2,448)

Initial panel 268 (131/137); replication panel 6,212 (2,996/3,216)

Affymetrix 406,177

Jiao et al [32]
Hindorff et al [18]

GWAS of body mass index

4,989: initial panel 1,715; replication panel 3,274

Initial panel 714 (354/360); replication panel 1,355

Initial panel 1,001 (545/456); replication panel 1,919

Affymetrix 746,626

Ng et al [33]
Hindorff et al [18]

GWAS of body mass index

249,796

107,795

140,490

102

Affymetrix, Illumina and Perlegen about 2.8 million (imputed)

Speliotes et al [34]
Hindorff et al [18]

Meta-analysis of extreme obesity

2,258

1,005

1,253

Affymetrix and Illumina 1,596,878 (imputed)

Scherag et al [35]
Hindorff et al [18]

GWAS of adult body mass index in a British population

9,023

4,487

4,536

Affymetrix GeneChip Mapping 500K
Illumina Infinium HumanHap550

Strachan et al [36]

GWAS of obesity

10,391

Randomly selected

Illumina 1,283,957 (imputed)

Dorajoo et al [37]
Hindorff et al [18]

Meta-analysis of GWAS informative for adult waist circumference and waist-hip ratio

140,644: initial panel 38,580; replication panel 102,064

Randomly selected

Affymetrix and Illumina 2,573,738 (imputed)

Lindgren et al [38]
Hindorff et al [18]

**Table 2.** List of Transcriptomic Data for Initial Data Set
Identification No.	Title	# Individuals	# Male	# Female	Age Min	Age max.	Analytical method	Refernce
GSE20950	Morbidly obese insulin-resistant patients: omental and subcutaneous adipose tissue	10	4	6	34	52	Affymetrix Human Genome U133 Plus 2.0 Array and real time PCR	Hardy et al [39]
GSE27951	Adipogenesis and obesity: subcutaneous adipose tissue (HG-U133_Plus_2)	14	10	4	45	49	Affymetrix Human Genome U133 Plus 2.0 Array	Keller et al [40]
GSE15524	Morbid obesity: subcutaneous and omental adipose tissues	11	2	9	37	47	CodeLink UniSet Human 20K I Bioarray	MacLaren et al [41]
GSE474	Obesity and fatty acid oxidation	12	0	12	32	41	Affymetrix Human Genome U133A Array	Park et al [42]
GSE15773	Obesity-associated insulin resistance independent of BMI: omental and subcutaneous adipose tissues	20	6	14	33	52	Affymetrix Human Genome U133 Plus 2.0 Array	Hardy et al [39]
GSE15653	Obese patients with and without type 2 diabetes: liver	13	4	9	28	58	Affymetrix Human Genome U133A Array	Pihlajamaki et al [43]
GSE22435	Expression of splicing factor genes is reduced in human obesity and contributes to enhanced lipogenesis	15	2	13	32	65	Affymetrix Human Genome U133 Plus 2.0 Array	Pihlajamaki et al [44]
GSE25401	Adipose tissue microRNAs as regulators of CCL2 production in human obesity (gene expression)	30	0	30	41	45	Affymetrix Human Gene 1.0 ST Array	Arner et al [45]
GSE25402	Adipose tissue microRNAs as regulators of CCL2 production in human obesity	30	0	30	41	45	Affymetrix Human Gene 1.0 ST Array (transcript (gene) version)	Arner et al [45]
GSE24883	Worsening of obesity and metabolic status yields similar molecular adaptations subcutaneous and visceral adipose tissue	24	0	24	33	56	Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)	Klimcakova et al [46]

Table 2. List of Transcriptomic Data for Initial Data Set

Identification No.

Title

# Individuals

# Male

# Female

Age Min

Age max.

Analytical method

Refernce

GSE20950

Morbidly obese insulin-resistant patients: omental and subcutaneous adipose tissue

Affymetrix Human Genome U133 Plus 2.0 Array and real time PCR

Hardy et al [39]

GSE27951

Adipogenesis and obesity: subcutaneous adipose tissue (HG-U133_Plus_2)

Affymetrix Human Genome U133 Plus 2.0 Array

Keller et al [40]

GSE15524

Morbid obesity: subcutaneous and omental adipose tissues

CodeLink UniSet Human 20K I Bioarray

MacLaren et al [41]

GSE474

Obesity and fatty acid oxidation

Affymetrix Human Genome U133A Array

Park et al [42]

GSE15773

Obesity-associated insulin resistance independent of BMI: omental and subcutaneous adipose tissues

Affymetrix Human Genome U133 Plus 2.0 Array

Hardy et al [39]

GSE15653

Obese patients with and without type 2 diabetes: liver

Affymetrix Human Genome U133A Array

Pihlajamaki et al [43]

GSE22435

Expression of splicing factor genes is reduced in human obesity and contributes to enhanced lipogenesis

Affymetrix Human Genome U133 Plus 2.0 Array

Pihlajamaki et al [44]

GSE25401

Adipose tissue microRNAs as regulators of CCL2 production in human obesity (gene expression)

Affymetrix Human Gene 1.0 ST Array

Arner et al [45]

GSE25402

Adipose tissue microRNAs as regulators of CCL2 production in human obesity

Affymetrix Human Gene 1.0 ST Array (transcript (gene) version)

Arner et al [45]

GSE24883

Worsening of obesity and metabolic status yields similar molecular adaptations subcutaneous and visceral adipose tissue

Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)

Klimcakova et al [46]